Principle:
nexttec™ 1^-Step Plant is the easiest handling and fastest DNA purification system containing a single buffer system and a 1^-Step DNA purification after lysis in 4 minutes. 

The nexttec™ 1^-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:

leaves tissue, plant seeds

Lysistime:

• 30 min to overnight

Amount of DNA:

• Up to 2,5 µg

Protocol for  Isolation of  Plant (Seed) using nexttec™ cleanPlates96:

Sample Lysis Transfer up to 20 mg seed meal, grist or a single crude squashed seed to a deep-well plate. Add 280 µl Buffer F, 20 µl Protease and 3 µl DTT. Incubate with shaking (60°C, 750 rpm, 30 min). Centrifuge the lysate (2,000x g, 3 min). Use clear supernatant for DNA purification.
1-Step DNA Purification Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlates96. Incubate for 3 min at room temperature. Centrifuge at 700x g for 1 min or apply vacuum for 1 min. The Eluate contains the Purified DNA!!

Ordering Informations:

Protocols:

47N-Plant_3.0_nexttec-cleanColumns.pdf

47N-Plant_3.0_nexttec-cleanPlate96.pdf