Principle:
nexttec™ 1-Step Blood (10 µl) is the easiest handling and fastest DNA purification system containing a single buffer system and a 1-Step DNA purification after lysis in 4 minutes.

The nexttec™ 1-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:
Fresh or frozen, EDTA, Heparin blood

Lysistime:

• 30 min to overnight

Amount of DNA:

• Up to 10 µg

Protocol for Isolation of Blood (10 µl) using nexttec™ cleanPlates96:

Sample Lysis Transfer 10 µl samples of EDTA or heparinized blood to a deep-well plate  Add 140 µl Buffer H, 10 µl Proteinase K to each sample.  Incubate with shaking (60°C, 200 rpm, 2h to overnight).
1-Step DNA Purification Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlates96. Incubate for 3 min at room temperature. Centrifuge at 700x g for 1 min or apply vacuum for 1 min. The Eluate contains the Purified DNA!!

Ordering Informations:

Protocols:

55N-Blood10_3.0_nexttec-cleanColumns.pdf

55N-Blood10_3.0_nexttec-cleanPlate96.pdf